Which solution will meet these requirements?

Explanations:

Configuring an S3 gateway endpoint allows EC2 instances in a VPC to access S3 directly without going through the internet, complying with the security regulations.

Creating an S3 bucket in a private subnet does not make sense, as S3 is a global service and does not reside in a VPC or subnet. Buckets cannot be private subnets.

While creating an S3 bucket in the same AWS Region as the EC2 instances is a good practice, it does not address the requirement of preventing internet traffic.

Configuring a NAT gateway allows instances in a private subnet to access the internet, which violates the company’s security regulations prohibiting internet traffic.